POS1169 THE INFLAMMATION INDUCED BY MONOSODIUM URATE AND CALCIUM PYROPHOSPHATE CRYSTALS DEPENDS ON OSMOLARITY AND AQUAPORIN CHANNELS.
نویسندگان
چکیده
Background The inflammation induced by monosodium urate (MSU) and calcium pyrophosphate (CPP) crystals is driven interleukin (IL)-1β production. This later relies on NLRP3 inflammasome which can be activated variation of ion concentration. Objectives To assess the role osmolarity water flux in MSU CPP crystal-induced inflammation. Methods In vitro, THP1 monocytes were stimulated pyrogen-free synthetic iso-, hypo- or hyperosmotic media. Cytokine production was quantified ELISA cell culture supernatants. Cell size measured using video microscopy. aquaporin channels assessed pharmacological inhibitor (mercury chloride, HgCl2). vivo, murine air pouch model used. injected mice treated not with HgCl2 mannitol. Osmolarity mouse sera patient synovial fluids (SF) freezing point osmometer. cells collected from SF imageJ software. Results IL-1β substantially reduced treatment (MSU 4900 vs 880 pg/ml; 10500 980, p<0.0001) when cultured medium. a transient increase 1.6 1.5 bigger after 30 100 min stimulation crystals, respectively. After 150 stimulation, decreased to their baseline size. abolished (assessed infiltration, CXCL2 lavage) drastically mannitol treatment. serum higher mannitol-treated than untreated (320 300 mmosm/L). patients, during flares had osteoarthitic SF. crystal-containing lower osteoarthritic (270 310 Finally, concentration strongly correlated osmolarity. Conclusion These results suggest that plays central Deciphering how modulate will identify new therapeutic targets. Disclosure Interests None declared
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Nucleation of monosodium urate crystals.
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The aim of this study was to evaluate whether DQ could prove useful to identify monosodium urate (MSU) and calcium pyrophosphate dehydrate (CPPD) crystals on permanent mounted stained slides. To this end, we studied 27 synovial fluid (SF) samples obtained from the knees of patients with the pseudogout (n=21) and acute gouty arthritis (n=6). Wet analysis for crystal detection and identification ...
متن کاملDiV Quik staining method for detection and identification of monosodium urate and calcium pyrophosphate crystals in synovial fluids
Objective—To evaluate whether the DiV Quik (DQ) staining method might prove useful in identifying monosodium urate (MSU) and calcium pyrophosphate dihydrate (CPPD) crystals on permanent mounted stained slides. Methods—27 synovial fluid (SF) samples obtained from the knees of 21 patients with acute CPPD disease and 6 with acute gout were studied. Wet analysis for crystal detection and identifica...
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Background. Urate crystals-induced inflammation is a critical factor during the initiation of gouty arthritis. Berberine is well known for its anti-inflammatory activity. However, the underlying effects of berberine on monosodium urate crystals-induced inflammation remain obscure. Objectives. This study is set to explore the protective effect and mechanism of berberine on monosodium urate cryst...
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ژورنال
عنوان ژورنال: Annals of the Rheumatic Diseases
سال: 2022
ISSN: ['1468-2060', '0003-4967']
DOI: https://doi.org/10.1136/annrheumdis-2022-eular.3571